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Figure 3 | BMC Biotechnology

Figure 3

From: A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines

Figure 3

Colony PCR and sequencing results for the pAAV-SirT1 gene KO plasmid . (a) Colony screening by PCR. Left arm (> 1 kb) and right arm (< 1 kb) positives are shown. (b) The sequence in the joint formed by the vector and the left arm of SirT1. The joint sequence is underlined in gray. (c) The sequence in the joint formed by the vector and the right arm of SirT1. The joint sequence is underlined in gray.

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BMC Biotechnology

ISSN: 1472-6750

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