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Table 4 Cell disruption conditions predicted by RSM and validation of the experimental data for cell disruption capability and specific protein release of HBsAg by optimal cell disruption strategy

From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

 

Optimal cell disruption strategy

Glass bead cell disruption

Parameters

  

Biomass concentration (g/L)

7.76

7.70

Number of passes (times)

20

-

Pulse pressure (bar)

1,029

-

Vortexing (times)

-

20

Glass beads (g/L)

-

40

Results

CDR (%)

75.68

37.18

Specific protein (mg/L)

29.20 ± 0.96

7.28 ± 1.06