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Table 4 Cell disruption conditions predicted by RSM and validation of the experimental data for cell disruption capability and specific protein release of HBsAg by optimal cell disruption strategy

From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

  Optimal cell disruption strategy Glass bead cell disruption
Biomass concentration (g/L) 7.76 7.70
Number of passes (times) 20 -
Pulse pressure (bar) 1,029 -
Vortexing (times) - 20
Glass beads (g/L) - 40
CDR (%) 75.68 37.18
Specific protein (mg/L) 29.20 ± 0.96 7.28 ± 1.06