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Table 2 Analysis of Variance (ANOVA) for quadratic model on the cell disruption capability and specific protein release activity of HBsAg from the result of CCD

From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

Source

DF

Sum of Squares

Mean Square

F value

p

Cell disruption capability

 Model

9

4946.52

549.61

11.53

0.0003

 A

1

24.63

24.63

0.52

0.4887

 B

1

68.42

68.42

1.44

0.2586

 C

1

3427.89

3427.89

71.9

< 0.0001

 A2

1

103.49

103.49

2.17

0.1714

 B2

1

307.23

307.23

6.44

0.0294

 C2

1

960.37

960.37

20.14

0.0012

 AB

1

54.44

54.44

1.14

0.3104

 AC

1

7.39

7.39

0.16

0.702

 BC

1

158.33

158.33

3.32

0.0984

 Residual

10

476.79

47.68

  

 Lack of Fit

5

453.91

90.78

19.85

0.0026

 Pure Error

5

22.87

4.57

  

Specific protein

 Model

9

1841

204.56

74.01

< 0.0001

 A

1

14.09

14.09

5.1

0.0476

 B

1

2.99

2.99

1.08

0.3228

 C

1

19.4

19.4

7.02

0.0243

 A2

1

537.7

537.7

194.56

< 0.0001

 B2

1

737.91

737.91

267

< 0.0001

 C2

1

872.46

872.46

315.68

< 0.0001

 AB

1

2.85E-04

2.85E-04

1.03E-04

0.9921

 AC

1

6.68

6.68

2.42

0.1511

 BC

1

2.85E-04

2.85E-04

1.03E-04

0.9921

 Residual

10

27.64

2.76

  

 Lack of Fit

5

19.77

3.95

2.51

0.1675

 Pure Error

5

7.87

1.57

 Â