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Table 2 Analysis of Variance (ANOVA) for quadratic model on the cell disruption capability and specific protein release activity of HBsAg from the result of CCD

From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

Source DF Sum of Squares Mean Square F value p
Cell disruption capability
 Model 9 4946.52 549.61 11.53 0.0003
 A 1 24.63 24.63 0.52 0.4887
 B 1 68.42 68.42 1.44 0.2586
 C 1 3427.89 3427.89 71.9 < 0.0001
 A2 1 103.49 103.49 2.17 0.1714
 B2 1 307.23 307.23 6.44 0.0294
 C2 1 960.37 960.37 20.14 0.0012
 AB 1 54.44 54.44 1.14 0.3104
 AC 1 7.39 7.39 0.16 0.702
 BC 1 158.33 158.33 3.32 0.0984
 Residual 10 476.79 47.68   
 Lack of Fit 5 453.91 90.78 19.85 0.0026
 Pure Error 5 22.87 4.57   
Specific protein
 Model 9 1841 204.56 74.01 < 0.0001
 A 1 14.09 14.09 5.1 0.0476
 B 1 2.99 2.99 1.08 0.3228
 C 1 19.4 19.4 7.02 0.0243
 A2 1 537.7 537.7 194.56 < 0.0001
 B2 1 737.91 737.91 267 < 0.0001
 C2 1 872.46 872.46 315.68 < 0.0001
 AB 1 2.85E-04 2.85E-04 1.03E-04 0.9921
 AC 1 6.68 6.68 2.42 0.1511
 BC 1 2.85E-04 2.85E-04 1.03E-04 0.9921
 Residual 10 27.64 2.76   
 Lack of Fit 5 19.77 3.95 2.51 0.1675
 Pure Error 5 7.87 1.57