| Variables | Cell disruption capability | Specific protein | ||||
---|---|---|---|---|---|---|---|
Experimental runs | Number of passes (times) | Biomass concentration (g/L) | Pulse pressure (bar) | CDR (%) | HBsAg (mg/L) | ||
 |  |  |  | Experimental | Predicted | Experimental | Predicted |
1 | 15(−1) | 5.74(−1) | 600(−1) | 53.83 | 56.04 | 8.74 | 8.96 |
2 | 25(+1) | 5.74(−1) | 600(−1) | 41.17 | 46.21 | 6.74 | 5.12 |
3 | 15(−1) | 9.65(+1) | 600(−1) | 27.64 | 37.44 | 10.99 | 9.92 |
4 | 25(+1) | 9.65(+1) | 600(−1) | 31.8 | 38.05 | 7.55 | 6.05 |
5 | 15(−1) | 5.74(−1) | 1400(+1) | 76.7 | 76.9 | 8.65 | 9.53 |
6 | 25(+1) | 5.74(−1) | 1400(+1) | 74.27 | 70.92 | 8.89 | 9.34 |
7 | 15(−1) | 9.65(+1) | 1400(+1) | 74.69 | 76.11 | 9.46 | 10.47 |
8 | 25(+1) | 9.65(+1) | 1400(+1) | 76.31 | 80.56 | 11.09 | 10.25 |
9 | 12(−α) | 7.70(0) | 1000(0) | 75.41 | 70.42 | 15.1 | 14.18 |
10 | 28(+α) | 7.70(0) | 1000(0) | 70.04 | 65.9 | 8.98 | 10.77 |
11 | 20(0) | 4.41(−α) | 1000(0) | 65.77 | 66.45 | 8.98 | 8.72 |
12 | 20(0) | 10.98(+α) | 1000(0) | 68.72 | 58.91 | 9.17 | 10.3 |
13 | 20(0) | 7.70(0) | 327.28(−α) | 36.75 | 26.01 | 3.68 | 5.74 |
14 | 20(0) | 7.70(0) | 1672.72(+α) | 77.68 | 79.3 | 10.94 | 9.75 |
15 | 20(0) | 7.70(0) | 1000(0) | 74.57 | 75.74 | 30.25 | 29.75 |
16 | 20(0) | 7.70(0) | 1000(0) | 72.24 | 75.74 | 29.39 | 29.75 |
17 | 20(0) | 7.70(0) | 1000(0) | 75.2 | 75.74 | 30.48 | 29.75 |
18 | 20(0) | 7.70(0) | 1000(0) | 75.62 | 75.74 | 28.29 | 29.75 |
19 | 20(0) | 7.70(0) | 1000(0) | 76.55 | 75.74 | 31.63 | 29.75 |
20 | 20(0) | 7.70(0) | 1000(0) | 78.69 | 75.74 | 28.62 | 29.75 |