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Table 1 Full factorial CCD matrix for the three significant variables and experimental and predicted values of cell disruption capability measured by CDR and specific protein release measured by ELISA for HBsAg concentration

From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

  Variables Cell disruption capability Specific protein
Experimental runs Number of passes (times) Biomass concentration (g/L) Pulse pressure (bar) CDR (%) HBsAg (mg/L)
     Experimental Predicted Experimental Predicted
1 15(−1) 5.74(−1) 600(−1) 53.83 56.04 8.74 8.96
2 25(+1) 5.74(−1) 600(−1) 41.17 46.21 6.74 5.12
3 15(−1) 9.65(+1) 600(−1) 27.64 37.44 10.99 9.92
4 25(+1) 9.65(+1) 600(−1) 31.8 38.05 7.55 6.05
5 15(−1) 5.74(−1) 1400(+1) 76.7 76.9 8.65 9.53
6 25(+1) 5.74(−1) 1400(+1) 74.27 70.92 8.89 9.34
7 15(−1) 9.65(+1) 1400(+1) 74.69 76.11 9.46 10.47
8 25(+1) 9.65(+1) 1400(+1) 76.31 80.56 11.09 10.25
9 12(−α) 7.70(0) 1000(0) 75.41 70.42 15.1 14.18
10 28(+α) 7.70(0) 1000(0) 70.04 65.9 8.98 10.77
11 20(0) 4.41(−α) 1000(0) 65.77 66.45 8.98 8.72
12 20(0) 10.98(+α) 1000(0) 68.72 58.91 9.17 10.3
13 20(0) 7.70(0) 327.28(−α) 36.75 26.01 3.68 5.74
14 20(0) 7.70(0) 1672.72(+α) 77.68 79.3 10.94 9.75
15 20(0) 7.70(0) 1000(0) 74.57 75.74 30.25 29.75
16 20(0) 7.70(0) 1000(0) 72.24 75.74 29.39 29.75
17 20(0) 7.70(0) 1000(0) 75.2 75.74 30.48 29.75
18 20(0) 7.70(0) 1000(0) 75.62 75.74 28.29 29.75
19 20(0) 7.70(0) 1000(0) 76.55 75.74 31.63 29.75
20 20(0) 7.70(0) 1000(0) 78.69 75.74 28.62 29.75
  1. () represents coded values.