Figure 6From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodologyWestern blot of cell disrupted samples using optimized conditions by high pressure homogenizer. Lane M represents protein marker, lane 1 represents cell disrupted sample. Western blot was performed using the same monoclonal anti-HBsAg as ELISA assay.Back to article page