Figure 4From: Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodologyTotal soluble protein (g/L) and selective product recovery (mg/g) of cell disruption process by the effects of three variables. Total soluble protein was measured using Bradford assay and selective product recovery was calculated based on the ratio of specific protein concentration measured by ELISA method to total soluble protein released. Experimental runs here mentioned are referred as in Table 1.Back to article page