Figure 2
From: Selective delivery of interleukine-1 receptor antagonist to inflamed joint by albumin fusion
Immuno-fluorescence staining detection for the receptor-binding of the fusion protein. A375.S2 cells incubated with 100 μg/ml fusion protein (1), with 10 μg/ml fusion protein (2), with 1 μg/ml fusion protein (3), with 0.1 μg/ml fusion protein (4), with 0 μg/ml fusion protein (5), and with 100 μg/ml HSA (6). FITC-labeled anti-His tag mAb is used in this assay. The fusion protein, IL-1ra and HSA have a His tag at the C-terminal.