Table 1 Plasmids used in this study
From: Structural insights from random mutagenesis of Campylobacter jejunioligosaccharyltransferase PglB
Plasmid | Description | Selection marker | Reference |
|---|---|---|---|
pACT3 | Medium copy number vector for IPTG-inducible expression, lacI, P tac , ori: pACYC184 | CmR | [25] |
pACT3Kan | Kanamycin resistance cassette inserted into chloramphenicol resistance gene of pACT3 | KanR | This study |
pGVXN114 | IPTG-inducible expression of PglB containing a hemagglutinin epitope tag (HA) at the C-terminal end, low copy number plasmid, lacI, P tac , ori: IncW | SpR | [4] |
pGVXN115 | Similar to pGVXN114, but inactive PglB-HA W458A-D459A (PglB mut) | SpR | [4] |
pGVXN112Kan | IPTG-inducible expression of PglB containing an HA tag at the C-terminal end, template plasmid for mutant libraries, medium copy number | KanR | This study |
pGVXN113Kan | Similar to pGVXN112Kan, but inactive PglB-HA W458A-D459A (PglB mut) | KanR | This study |
pGVXN150 | L-arabinose inducible expression of periplasmic, hexahistidine-tagged Pseudomonas aeruginosa exotoxoid A (EPA) with 2 engineered N-glycosylation sites, araC, P BAD , high copy number plasmid, ori: pBR322 | AmpR | [4] |
pGVXN345 | Constitutive expression of CP5 capsular polysaccharides of Staphyloccus aureus, native promoter, low copy number plasmid, ori: IncPa | TetR, CmR | M. Wacker and J. Lee, in preparation |