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Figure 6 | BMC Biotechnology

Figure 6

From: Structural insights from random mutagenesis of Campylobacter jejunioligosaccharyltransferase PglB

Figure 6

Structural context of V432 and the MIV motif. (A) Alignment of bacterial PglB sequences in the vicinity of C. jejuni V432 and I571/V575. Numbering of ruler according to PglB C. lari , conserved residues shaded, residues shown in B marked by arrows. (B) Position of residues equivalent to PglB Cj V432, I571 and V575 in the crystal structure 3RCE of PglB C.lari [11] (red). Periplasmic domain in grey, transmembrane domain in dark green, bound D-Q-N-A-T- p-nitrophenylalanine acceptor peptide as atom-coloured ball-stick representation, Mg2+ ion as light-green sphere.

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