Figure 3
From: Structural insights from random mutagenesis of Campylobacter jejunioligosaccharyltransferase PglB
Analysis of PglB expression by anti-HA Western blot. Selected inactive clones of epPCR libraries (PglB mutations given in lane header) and control strains were grown and induced in DWP. wt: pGVXN112Kan, mut: pGVXN113Kan, host: E. coli CLM24 (pGVXN150, pGVXN345). Relative expression levels of full-length PglB given in subsequent Figures and Tables were determined from the upper, boxed bands by image analysis with the software ImageJ (wt PglB = 100%, S = strong, W = weak, N = negative).