Table 3 Amount of active E1 (μg/g FW) in infiltrated N. benthamiana leaves using the different CMVin and CMVar leader sequence variants

1.09 ± 0.20^1D

−0.01 ± 0.00^D

14.74 ± 2.14^A

13.97 ± 3.40^A

1.07 ± 0.80^E

0.22 ± 0.36^E

8.76 ± 3.49^BC

9.38 ± 2.10^BC

1.00 ± 0.45^D

0.76 ± 0.11^D

10.05 ± 1.28^B

11.05 ± 3.85^B

2.23 ± 0.59^CD

0.00 ± 0.01^D

0.10 ± 0.08^D

−0.01 ± 0.00^D

6.30 ± 3.50^CD

0.01 ± 0.03^E

0.33 ± 0.33^E

0.05 ± 0.12^E

10.78 ± 3.63^BC

−0.01 ± 0.01^D

1.58 ± 0.49^D

1.85 ± 1.13^D

4.12 ± 3.35^CD

0.00 ± 0.01^D

0.67 ± 0.36^D

−0.01 ± 0.00^D

9.38 ± 2.10^AB

0.00 ± 0.01^E

0.57 ± 0.45^E

0.38 ± 0.79^E

15.15 ± 5.39^AB

0.39 ± 0.30^D

1.88 ± 0.32^D

1.28 ± 0.53^D

6.74 ± 2.82^BC

0.08 ± 0.12^D

1.17 ± 0.82^D

2.27 ± 1.81^D

5.61 ± 2.01^CDE

0.01 ± 0.01^E

1.87 ± 0.64^DE

1.15 ± 0.77^DE

14.06 ± 2.71^AB

0.21 ± 0.26^D

1.17 ± 0.98^CD

1.72 ± 0.93^CD

11.34 ± 2.67^AB

1.42 ± 0.33^D

2.26 ± 0.36^CD

1.31 ± 0.81^CD

19.60 ± 4.56^A

1.49 ± 0.14^DE

2.03 ± 0.31^CE

1.73 ± 1.09^DE

20.99 ± 5.18^A

1.16 ± 0.96^D

1.97 ± 0.48^D

2.49 ± 1.61^D