Bipartite and tripartite Cucumber mosaic virus-based vectors for producing the Acidothermus cellulolyticus endo-1,4-β-glucanase and other proteins in non-transgenic plants

Table 2 Nomenclature and composition of CMV-based systems

Leader sequences in mRNA4	Protein encoded by RNA3¹	Genotype of RNA 1 and 2²	Replicating (R) or inducible(I)	Name of combined CMV variants
wt	CP	Subgroup I	R	CMVarI wt
2				CMVarI 2
6				CMVarI 6
8				CMVarI 8
wt	CP	Subgroup II	R	CMVarII wt
2				CMVarII 2
6				CMVarII 6
8				CMVarII 8
wt	CP	Subgroup II	I	CMVinII wt
2				CMVinII 2
6				CMVinII 6
8				CMVinII 8
wt	GFP	Subgroup I	R	CMVarI Gwt
2				CMVarI G2
6				CMVarI G6
8				CMVarI G8
wt	GFP	Subgroup II	R	CMVarII Gwt
2				CMVarII G2
6				CMVarII G6
8				CMVarII G8
wt	GFP	Subgroup II	I	CMVinII Gwt
2				CMVinII G2
6				CMVinII G6
8				CMVinII G8
wt	E1	Subgroup I	R	CMVarI Ewt
2				CMVarI E2
6				CMVarI E6
8				CMVarI E8
wt	E1	Subgroup II	R	CMVarII Ewt
2				CMVarII E2
6				CMVarII E6
8				CMVarII E8
wt	E1	Subgroup II	I	CMVinII Ewt
2				CMVinII E2
6				CMVinII E6
8				CMVinII E8
wt	GFP	Subgroup I	R	CMVarI 33Gwt
2				CMVarI 33 G2
6				CMVarI 33 G6
8				CMVarI 33 G8
wt	GFP	Subgroup II	R	CMVarII 33Gwt
2				CMVarII 33 G2
6				CMVarII 33 G6
8				CMVarII 33 G8
wt	GFP	Subgroup II	I	CMVinII 33Gwt
2				CMVinII 33 G2
6				CMVinII 33 G6
8				CMVinII 33 G8
wt	E1	Subgroup I	R	CMVarI 33Ewt
2				CMVarI 33E2
6				CMVarI 33E6
8				CMVarI 33E8
wt	E1	Subgroup II	R	CMVarII 33Ewt
2				CMVarII 33E2
6				CMVarII 33E6
8				CMVarII 33E8
wt	E1	Subgroup II	I	CMVinII 33Ewt
2				CMVinII 33E2
6				CMVinII 33E6
8				CMVinII 33E8

¹All RNA3 constructs are from subgroup II CMV-Q. ²RNA 1 and 2 sources are subgroup I or subgroup II as indicated. Cells containing required plasmids were mixed and infiltrated into non-transgenic N. benthamiana plants as described.

ISSN: 1472-6750