Figure 4

Immunoblot analysis of E1 produced in infiltrated N. benthamiana leaves. N. benthamiana leaves were co-infiltrated with A. tumefaciens containing plasmids corresponding to the CMVinII E and CMVarII E variants (see Table 2) shown. CMVinII E leaves were treated with estradiol 18 hrs after infiltration. Leaves were harvested 6 days post-infiltration. Soluble proteins were extracted as described in Methods and concentrations were determined by Bradford assay. Numbers above each lane correspond to the specific RNA 3 variant used. Each sample represents 15 μg total protein extract per lane. Prestained size marker (Benchmark, Invitrogen, Carlsbad, CA, U.S.A.) shows 80 kDa band.