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Figure 1 | BMC Biotechnology

Figure 1

From: Bipartite and tripartite Cucumber mosaic virus-based vectors for producing the Acidothermus cellulolyticus endo-1,4-β-glucanase and other proteins in non-transgenic plants

Figure 1

(A) Diagram of the E1 constructs and (B) Modified RNA 4 leader sequences containing restriction enzyme sites compared to unmodified wild type (wt) leader sequences. (A) Gene structure of the E1 used in this report. The E1 gene contains the rice amylase signal peptide in the upstream of its ORF and a 6 histidine-tag in its C-terminus. RAmySP; rice amylase signal sequence. This Figure is not to scale. (B) Restriction endonuclease sequences are shown as italicized and underlined, the CP start codon (AUG) is shown in bold. Modified leader sequences are located between the wildtype leader sequence and AUG start codon in subgenomic RNA4. Wt, wildtype leader sequence; 2+, modified #2 leader sequences addition to the wildtype leader sequence; 6+, modified #6 leader sequences addition to the wildtype leader sequence; 8+, modified #8 leader sequences addition to the wildtype leader sequence.

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BMC Biotechnology

ISSN: 1472-6750

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