Q-PCR analysis of the isolated clones. (A) GIP mRNA expression was higher in the five isolated K-cell clones than in STC-1 cells as a control. (B) GLP-1 mRNA expressions was higher in the five isolated L-cell clones than in STC-1 cells as control. BioRad CFX Manager Software was used to analyze expression data. Gene expression was normalized for mouse β-actin and β-2 microglobulin (β2m) mRNA expression. Error bars indicate standard deviation.