Figure 4

SDS-PAGE of eluted and purified GST-GFP. A: SDS PAGE image of proteins obtained from (lane 1) crude soluble lysate, (lane 2) after Ni purification, (lane 3) Ni purification in the presence of 10 mM glutathione, (lane 4) purification with PEGDA gel homogenate, and (lane 5) purified with PEGDA:GSH homogenate. Blue arrows indicate 100 kDa and 55 kDa while the green and red arrows are the proposed GST-GFP monomer and dimer, respectively. B: Protein recovered from PEGDA or PEGDA:GSH gel homogenates calibrated to total protein concentration from lanes 4 and 5 of panel. Data is presented the mean plus or minus (±) standard deviation.