Figure 2

Dependence of N2a cell adhesion on protein coating concentration. Protein-coated surfaces were prepared using 0.1, 0.2, 0.5, 1, 2, 5 or 10 μM of [V₇]₂₀, [RGD-V₆]₂₀ and fibronectin. Protein concentration was prepared based on the calculated MWs of 58.2, 59.6 and 225 kDa for [V₇]₂₀, [RGD-V₆]₂₀ and fibronectin. A₄₀₅ values of samples were subtracted by 0.035 ± 0.004 of background control and used to generate cell adhesion graphs. * and # correspond to P < 0.05 and P < 0.01 from [V₇]₂₀, respectively.