From: In vivo functional expression of a screened P. aeruginosa chaperone-dependent lipase in E. coli
Primers | Sequence (5’-3’) | Source |
---|---|---|
A-F | GGATCCATGAAGAAGAAGTCTCTGCT | Amplification of lipA, BamHI site |
A-R | AAGCTTCTACAGGCTGGCGTTCTT | Amplification of lipA, HindIII site |
B-F | GGGCATATGGTGAAGAAAATCCTCCTG | Amplification of lipB, NdeI site |
B-R | CTCGAGTCAGCGCTGCTCGGCCT | Amplification of lipB, XhoI site |
AB-F | CCTTGGATCCATGAAGAAGAAGTCTCTGCTCC | Amplification of lipAB, BamHI site |
AB-R | CCTTAAGCTTCAGCTGCTCGGCCTGGCGCAT | Amplification of lipAB, HindIII site |