Figure 3From: High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion systemOne-step purification of TF AAA -USP18. TFAAA-USP18 was bound to a Co-IMAC column and eluted with imidazole. Purity of the eluted fusion protein was visualized by SDS-PAGE with subsequent Coomassie staining.Back to article page