Skip to main content

Table 1 Starting bacterial strains and plasmids used in this study

From: Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061

Strain or plasmid Description Source
Strains
E. coli TOP10 lacх74 recA1 deoR F – mcrA Δ (mrr-hsdRMS-mcrBC) ϕ80 lacZΔM15Δ araD139Δ (ara-leu)7697 galU galK Transgen
E. coli BL21(DE3) F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr Novagen
Fusarium proliferatum LF061 Wild strain, producing beauvericin isolated from marine sample This study
E. coli TOP10-KivRFp Positive clone of artificial gene synthesis, which carries the KivRFp gene fragment This study
E. coli BL21(DE3)-KivRFp Positive clone, which carries the pKivRFp-His expression vector This study
Plasmids
pUCE Cloning vector; Apr Inovogen
pET28a Expression vector; Kmr Novagen
pUCE-KivRFp pUCE, which carries the complete ketoisovalerate reductase (KivRFp) This study
pKivRFp-His pET28a carrying amplified EcoRI-NdeI fragment containing ketoisovalerate reductase gene (KivRFp) This study
  1. Apr, ampicillin resistant; Kmr, kanamycin resistant.