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Strains
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E. coli TOP10
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lacх74 recA1 deoR F – mcrA Δ (mrr-hsdRMS-mcrBC) ϕ80 lacZΔM15Δ araD139Δ (ara-leu)7697 galU galK
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Transgen
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E. coli BL21(DE3)
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F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr
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Novagen
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Fusarium proliferatum LF061
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Wild strain, producing beauvericin isolated from marine sample
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This study
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E. coli TOP10-KivRFp
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Positive clone of artificial gene synthesis, which carries the KivRFp gene fragment
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This study
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E. coli BL21(DE3)-KivRFp
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Positive clone, which carries the pKivRFp-His expression vector
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This study
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Plasmids
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pUCE
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Cloning vector; Apr
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Inovogen
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pET28a
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Expression vector; Kmr
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Novagen
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pUCE-KivRFp
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pUCE, which carries the complete ketoisovalerate reductase (KivRFp)
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This study
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pKivRFp-His
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pET28a carrying amplified EcoRI-NdeI fragment containing ketoisovalerate reductase gene (KivRFp)
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This study
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