Table 1 Starting bacterial strains and plasmids used in this study

Strains

E. coli TOP10

lacх74 recA1 deoR F – mcrA Δ (mrr-hsdRMS-mcrBC) ϕ80 lacZΔM15Δ araD139Δ (ara-leu)7697 galU galK

Transgen

E. coli BL21(DE3)

F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr

Novagen

Fusarium proliferatum LF061

Wild strain, producing beauvericin isolated from marine sample

This study

E. coli TOP10-KivRFp

Positive clone of artificial gene synthesis, which carries the KivRFp gene fragment

This study

E. coli BL21(DE3)-KivRFp

Positive clone, which carries the pKivRFp-His expression vector

This study

Plasmids

pUCE

Cloning vector; Ap^r

Inovogen

pET28a

Expression vector; Km^r

Novagen

pUCE-KivRFp

pUCE, which carries the complete ketoisovalerate reductase (KivRFp)

This study

pKivRFp-His

pET28a carrying amplified EcoRI-NdeI fragment containing ketoisovalerate reductase gene (KivRFp)

This study