Figure 5From: Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061Substrate specificity of overexpressed and purified ketoisovalerate reductase KivRFp. 5A). Specific activity of reductive reaction of different homologous compounds using NADPH (0.29 mM) as coenzyme. 2-ketoisovalerate (2-Kiv), 2-ketovalerate (2-Kva), pyruvate, 2-ketocapronate (2-Kca), 2-ketoglutarate (2-Kgl), and 2-ketobutyrate (2-Kbu). 5B). Specific activity of reductive reaction of different coenzymes using 2-Kiv (0.7 mM) as substrate. Relative activity was shown as the percentage of the activity of the activity towards 2-ketoisovalerate. All measurements were performed in triplicate.Back to article page