Expression of protein in both bacteria and yeast from the pDEPC plasmids. (A) Comparison protein expression levels from the CUP1 and MET25 promoters in yeast by Western blot analysis of yeast lysates using α-GST antibodies of GST-MVB12 fusion protein (38KDa). Left panel, protein expression from pC-DEPC-MVB12 plasmid under the control of the CUP1 promoter was induced by the addition of copper chloride; and right panel is same protein expressed under control of the MET25 promoter using pM-DEPC-MVB12 plasmid, induced by the absence of methionine. (B) Comparison of protein expression levels from the indicated E. coli strains transformed with the pC-DEPC-MVB12 plasmid. Lanes are labeled U for uninduced and I for induced with 0.2 mM IPTG for 2 hrs. Bacteria lysates were separated by SDS-PAGE gel stained by Coomassie Brilliant Blue. White circles in induced lanes indicate the GST-MVB12 fusion protein (38 kDa).