Schematic representation of pDEPC vectors. (A) Schematic of pC-DEPC and pM-DEPC vectors with the following features labeled: URA3 ORF in blue, f1 ori in white, CUP1 yeast promoters in green, MET25 yeast promoters in orange, glutathione S-transferase gene (GST) in yellow, the CYC1 yeast transcription terminator in red, the pBM1 origin of replication in white, and the bla gene conferring ampicillin resistance in grey. Unique restriction sites are labeled. (B) Nucleotide sequence of the translation initiation region and corresponding protein translation are shown. This region positions the prokaryotic T7 promoter and ribosomal binding site (RBS) after the eukaryotic CUP1 or MET25 promoters. A TEV protease cleavage site is located between the GST-tag and the insertion site for the ORF of interest. (C) Nucleotide sequence of the multiple cloning site (MCS).