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Table 3 Strains and plasmids used in this study

From: Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

Strain or plasmid Relevant characteristics References
Strains
P. putida KT2440 Prototrophic, reference strain http://openwetcare.org/wiki/E._coli_genotypes
E. coli W3110 Wild type, xylAB donor http://openwetcare.org/wiki/E._coli_genotypes
E. coli JM109 endA1, glnV44, gyrA96, thi-1, mcrB+, hsdR17 (rk, mk+), relA1, supE44, [F' traD36 proAB+lacIqlacZΔM15] http://openwetcare.org/wiki/E._coli_genotypes
E. coli HB101 F-, hsdS20 (rB- mB-) recA13, ara-14, proA2, lacY1, galK2, xyl-5, mtl-1, rpsL20 (SmR). http://openwetcare.org/wiki/E._coli_genotypes
Plasmids   
RK600 Cmr, ColE1, oriV, RK2, mob+, tra+ [38]
pVLT33 Kmr, Ptac, MCS of pUCP18, hybrid broad-host-range expression vector [39]
pSLM1 xylA and xylB cloned into pVLT33 this study
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