Table 3 Strains and plasmids used in this study

Strains

P. putida KT2440

Prototrophic, reference strain

http://openwetcare.org/wiki/E._coli_genotypes

E. coli W3110

Wild type, xylAB donor

http://openwetcare.org/wiki/E._coli_genotypes

E. coli JM109

endA1, glnV44, gyrA96, thi-1, mcrB⁺, hsdR17 (r_k^–, m_k⁺), relA1, supE44, [F' traD36 proAB⁺lacI^qlacZΔM15]

http://openwetcare.org/wiki/E._coli_genotypes

E. coli HB101

F^-, hsdS20 (r_B^- m_B^-) recA13, ara-14, proA2, lacY1, galK2, xyl-5, mtl-1, rpsL20 (Sm^R).

http://openwetcare.org/wiki/E._coli_genotypes

Plasmids

RK600

Cm^r, ColE1, oriV, RK2, mob+, tra+

[38]

pVLT33

Km^r, Ptac, MCS of pUCP18, hybrid broad-host-range expression vector

[39]

pSLM1

xylA and xylB cloned into pVLT33

this study