Skip to main content

Table 3 Strains and plasmids used in this study

From: Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

Strain or plasmid Relevant characteristics References
P. putida KT2440 Prototrophic, reference strain
E. coli W3110 Wild type, xylAB donor
E. coli JM109 endA1, glnV44, gyrA96, thi-1, mcrB+, hsdR17 (rk, mk+), relA1, supE44, [F' traD36 proAB+lacIqlacZΔM15]
E. coli HB101 F-, hsdS20 (rB- mB-) recA13, ara-14, proA2, lacY1, galK2, xyl-5, mtl-1, rpsL20 (SmR).
RK600 Cmr, ColE1, oriV, RK2, mob+, tra+ [38]
pVLT33 Kmr, Ptac, MCS of pUCP18, hybrid broad-host-range expression vector [39]
pSLM1 xylA and xylB cloned into pVLT33 this study