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Table 2 PHA production in batch and fed-batch fermentations

From: Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440

Entry Strains Fermentation type Carbon source Feeding type/duration Feeding phase Flow Total amount of fed octanoic acid OD600 μmax (h-1) PHA content (% w w-1)
    growth substrate PHA precusor        
A KT2440 (pVLT33) Batch xylose - - - -   no growth - -
B KT2440 (pSLM1) Batch xylose - - - -   0.99 0.24 0.3
C KT2440 (pSLM1) Batch octanoic acid - - - - 1.44 g L-1 2.77 0.35 21.0
D KT2440 (pSLM1) Fed-Batch xylose octanoic acid linear/12 h mid exp. (1) 0.072 g L-1 h-1 0.87 g L-1 1.48 0.24 12.1
E KT2440 (pSLM1) Fed-Batch xylose octanoic acid linear/8 h end exp. (2) 0.072 g L-1 h-1 0.58 g L-1 1.52 0.25 16.2
F KT2440 (pSLM1) Fed-Batch xylose octanoic acid linear/4 h end exp. (2) 0.288 g L-1 h-1 1.15 g L-1 1.4 0.25 20
G KT2440 (pSLM1) Batch xylose + octanoic acid octanoic acid - - - 1.44 g L-1 2.13 0.27 28.7
  1. The cells were grown in 0.2NE2 minimal media supplemented with different carbon sources in 1 L mini-bioreactors with a working volume of 800 mL. Samples were taken regularly to measure growth, nitrogen concentration, carbon source present in the medium and PHA contents. Data points are the averages of duplicate measurements.
  2. (1): Feeding started at the middle of exponential batch phase, OD600 of 0.5.
  3. (2): Feeding started at the end of exponential batch phase, OD600 of 1.
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