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Table 1 Percent hydroxylation of proline in bovine collagen III and in recombinant human collagen III

From: Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

  LC-MS Assay (%) Amino Acid Analysis (%)
Bovine collagen III (as-received) 38 ± 1.1 37 ± 2.7
Bovine collagen III (extracted from SDS-PAGE) 36 ± 3.4 n/a
Recombinant human collagen III N.D.* N.D.*
  No hydroxylase (p4Ha, p4Hb) genes
  Collagen on 2-micron plasmid
Recombinant human collagen III 5.8 ± 0.5 5.2 ± 1.3
  CEN/ARS–p4Ha–p4Hb with SUC2 signal
  Collagen on 2-micron plasmid
Recombinant human collagen III 12 ± 1.8 12§
  CEN/ARS–p4Ha–p4Hb with SUC2 signal
  Collagen on CEN/ARS plasmid
  1. * "N.D." denotes no significant signal of hydroxyproline above background noise.
  2. §Only 1 AAA result available.