Table 1 Percent hydroxylation of proline in bovine collagen III and in recombinant human collagen III

Bovine collagen III (as-received)

38 ± 1.1

37 ± 2.7

Bovine collagen III (extracted from SDS-PAGE)

36 ± 3.4

n/a

Recombinant human collagen III

N.D.*

N.D.*

  No hydroxylase (p4Ha, p4Hb) genes

  Collagen on 2-micron plasmid

Recombinant human collagen III

5.8 ± 0.5

5.2 ± 1.3

  CEN/ARS–p4Ha–p4Hb with SUC2 signal

  Collagen on 2-micron plasmid

Recombinant human collagen III

12 ± 1.8

12^§

  CEN/ARS–p4Ha–p4Hb with SUC2 signal

  Collagen on CEN/ARS plasmid