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Figure 2 | BMC Biotechnology

Figure 2

From: Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

Figure 2

Reconstructed ion chromatograms of the hydrolyzed collagen samples. Samples were separated by reversed-phase liquid chromatography and detected by ESI mass spectrometry. (A) Bovine collagen III, as received, (B) Bovine collagen III extracted from SDS-PAGE, and (C) Recombinant human collagen III. Rows: Top, IS = internal standard; Middle, HYP = hydroxyproline; Bottom, PRO = proline.

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