An improved method for isolation of RNA from bone

BMC Biotechnology

Table 1 RNA Yield and Quality Using a One-Step Approach to Extract RNA from Bone

	Yield (μg)	A260/280	A260/230	RIN
one-step	26.0 -/+ 7.76	2.09 -/+ 0.015	2.17 -/+ 0.163	8.28 -/+ 0.87
two-step	3.17 -/+ 3.04	1.91 -/+ 0.155	1.09 -/+0.760	5.65 -/+ 2.15

RNA yield is reported based on the absorbance at 260 nm using a Nanodrop spectrophotometer and confirmed using the Agilent RNA 6000 Nano LabChip Kit and the Agilent 2100 Bioanalyzer. RNA quality is reported as the ratio of the absorbance at 260 nm and 280 nm along with the ratio of the absorbance at 260 nm and 230 nm as well as the RNA Integrity Number based on the entire electrophoretic RNA sample trace produced using the Agilent 2100 Bioanalyzer. Each value is reported at the mean -/+ standard deviation.

ISSN: 1472-6750