Purification steps | Total volume (mL) | Total enzyme activity (U) | Total Protein content (mg) | Specific activity (U/mg) | Purification fold | % Yield |
---|---|---|---|---|---|---|
Crude enzyme | 200 | 118400 | 698 | 170 | 1 | 100 |
(NH4)2SO4 precipitation | 25 | 13375 | 54 | 248 | 1.5 | 11.3 |
Sephadex-G-100 | 12 | 5724 | 13 | 440 | 2.6 | 4.8 |
DEAE-Cellulose | 9 | 3870 | 7 | 553 | 3.3 | 3.2 |