Skip to main content

Table 4 Effect of increasing cells on cell survival and transfection efficiency using the cell line L kit and T030 program

From: Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3

Program

Cells

pmaxGFP (μg)

% Cell survival

% Transfection efficiency

% Cell input, alive and transfected

T-030

500,000

No GFP control

36.0 ±4.2*

0.4 ±0.2*

0.1

T-030

500,000

3 μg

19.0 ±0.8*

61.3 ±5.5*

11.6

T-030

1,000,000

3 μg

13.0 ±1.0#

88.5 ±2.3#

11.5

T-030

1,500,000

3 μg

11.0 ±1.2#

89.4 ±2.0#

9.8

  1. Values ± SD with * represent the mean of 4 independent experiments, each with 4 biological replicates and values with # represent the mean of 2 independent experiments, each with 2 biological replicates.