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Table 3 Effect of increasing plasmid concentration on cell survival and transfection efficiency using kit-L and the T030 program

From: Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3

Program pmaxGFP (μg) % Cell survival % Transfection efficiency
T-030 No GFP control 36.0 ±4.2* 0.7 ±0.2*
T-030 3 μg 19.0 ±1.2* 60.2 ± 6.7*
T-030 6 μg 13 90.8
T-030 9 μg 10 81.4
  1. Values ± SD with * represent the mean of 3 independent experiments, each with 3 biological replicates. The other means are derived from one experiment with 3 biological replicates.