Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3

Table 3 Effect of increasing plasmid concentration on cell survival and transfection efficiency using kit-L and the T030 program

Program	pmaxGFP (μg)	% Cell survival	% Transfection efficiency
T-030	No GFP control	36.0 ±4.2*	0.7 ±0.2*
T-030	3 μg	19.0 ±1.2*	60.2 ± 6.7*
T-030	6 μg	13	90.8
T-030	9 μg	10	81.4

Values ± SD with * represent the mean of 3 independent experiments, each with 3 biological replicates. The other means are derived from one experiment with 3 biological replicates.

ISSN: 1472-6750