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Table 2 Effect of specific nucleofection programs on cell survival and transfection efficiency of human primary calvarial suture mesenchymal cells

From: Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3

  

V- Nucleofector buffer

L- Nucleofector buffer

Nucleofection program

pmaxGFP (μg)

% Cell survival

% Transfection efficiency

% Cell survival

% Transfection efficiency

No-Program control

3μg

99.2

0.2

98.8±0.7*

0.4±0.1*

T-030

No GFP control

26

0.3

37.0 ±7.0*

0.6±0.2*

T-030

3μg

14

35.5

19.0±2.0*

56.2±9.1*

A-020

3 μg

20

10.4

24

3.8

T-020

3 μg

14

22.7

17

31.6

X-001

3 μg

24

12.8

27

8.0

X-005

3 μg

24

16.5

20

13.7

L-029

3 μg

11

23.5

18

22.3

D-023

3 μg

15

27.2

20

14.8

  1. Values ± SD with * represent the mean of 2 independent experiments, each with 2 from biological replicates. The other values are derived from one experiment with 2 biological replicates.