Figure 5From: Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100Comparison of endogenous ApoB mRNA knockdown by shApoB1, shApoB2, miApoB1 and miApoB2in Hepa1-6 cells. Endogenous ApoB mRNA knockdown by H1-shApoB1, CMV-shApoB1 and CMV-miApoB1 constructs (b) Endogenous ApoB mRNA knockdown by H1-shApoB2, CMV-shApoB2 and CMV-miApoB2 constructs qRT-PCR was performed two days post-transfection with 1 μg of shRNA or miRNA expressing constructs and ApoB mRNA levels were calculated relative to actin mRNA. H1-shApoB1 and H1-ApoB1 served as positive control. shScr and miScr served as negative controls and were set at 100%. Data are represented as mean values + SD from three independent experiments analyzed with factor correction method [19]. **p < 0.01 versus negative control (One-way ANOVA test with Bonferroni post test).Back to article page