Experimental field trial (T plot). A) Description of all of the citrus lines selected for release in the experimental orchard, including the citrus type, genetic modification (GM), developmental stage (ontogeny), ploidy level and number of plants of each line. T, transgenic; C, control; J, juvenile; A, adult; 2n, diploid; 4n, tetraploid. B) Images showing the T plot in 1998 (left) and in 2004 (right). C) Schematic diagram of the T plot showing the arrangement of the 130 trees, including 16 transgenic plants of Pineapple sweet orange (green), 16 transgenic plants of Carrizo citrange (blue) and 16 transgenic plants of Mexican lime (red). In addition, there were 8 non-transgenic control plants from each citrus type interspersed individually between the two plants from each transgenic line (black). Fifty-eight non-transgenic Clemenules clementine trees planted along an external edge (white circles) were used as a buffer to prevent transgene flow through pollen dispersal.