Figure 6From: Development of an optimized tetracycline-inducible expression system to increase the accumulation of interleukin-10 in tobacco BY-2 suspension cellsComparison of constitutive and inducible vIL-10 accumulation in BY-2 cell suspension cultures. Samples were taken from cultures expressing vIL-10 constitutively (cells transformed with pTRAkt_ER-vIL-10, black bars) at 3, 5 and 7 days post subculture. Samples were taken from cultures expressing vIL-10 under induction (cells transformed with pTRAh_DsRed-eTXvIL-10, grey bars) daily from 3 to 7 days post induction. The accumulation of vIL-10 was determined in cell pellet extracts by ELISA. The data reported are expressed as μg/g FW and represent the means ± SD from three independent experiments.Back to article page