Figure 2

Schematic overview of the T-DNA cassettes used for constitutive and inducible expression of vIL-10. LB and RB, left and right border of the T-DNA; P35STX, Inducible Cauliflower mosaic virus 35 S promoter; Pe35S, CaMV 35 S promoter with enhancer (double arrowheads); TX, three tetO sites (TetR binding sites, gray circle) surrounding the TATA box; OCS, octopine synthase gene terminator; pAg7, agropine synthase gene terminator; Pnos and pAnos, nopaline synthase gene promoter and terminator; hpt, hygromycin phosphotransferase gene; nptII, neomycin phosphotransferase gene; SAR, scaffold attachment region; pA, terminator of the CaMV 35 S gene; TL, 5′ UTR of the Tobacco etch virus; vIL-10, viral IL-10 coding sequence with native signal peptide sequence at the 5′ end and sequences for a His₆ tag and ER retention signal (SEKDEL) at the 3′ end; TP, plastid transit peptide from the barley granule-bound starch synthase gene; DsRed, Discosoma spp red fluorescent protein. The full name of the vector is shown to the side of each diagram and the vector backbone and promoter are indicated in parentheses. Not drawn to scale.