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Table 6 Results of the ligation comparison using chemicals from PPLMD I protocol with different cycle conditions

From: Comparison and transfer testing of multiplex ligation detection methods for GM plants

    ΔCt (SD)  
Target GM mixes Target % PPLMD I 65°C DTM 65°C LPA
H60-L 54°C
PPLMD II
65°C
SNPlex Ramp 70-61°C p-value ANOVA
hmg 1 100 14.3 (0.4) a 10.0 (0.7) c 9.0 (1.4) c 12.0 (0.2)b 12.6 (0.7)ab 1.2E-06
  2 100 13.8 (0.9) a 10.5 (1.0) cd 9.3 (0.9) d 12.6 (0.7) ab 12.0 (0.2) bc 6.5E-06
  3 5 7.8 (1.6) 5.2 (1.3) 4.7 (2.2) 7.5 (1.1) 7.1 (0.2) > 0.01
bar 1 0.1 ND  
  2 2.5 3.9 (0.5) 2.5 (0.7) 4.5 (3.19) 4.0 (0.7) 3.2 (1.0) > 0.01
  3 2.5 3.5 (1.2) 2.5 (1.3) 3.4 (3.5) 3.3 (1.3) 3.9 (0.7) > 0.01
cry1Ab 1 0.1 ND  
  2 2.5 7.5 (0.0) b 5.2 (0.5) b 4.4 (2.5) b 11.4 (0.6) a 7.5 (2.3) b 1.2E-04
  3 2.5 6.9 (0.6) b 5.7 (0.5) b 5.4 (2.4) b 10.9 (0.3) a 7.5 (2.1) b 5.7E04
TC 1507 1 5 ND  
  2 1 ND  
  3 2.5 ND  
  1. SD: standard deviation.
  2. Values marked with different superscript characters indicate groups (for the same target-mix combination) that are significantly different according to Tukeys HSD test (P < 0.05); xa is different from yb, zab is not different from either.
  3. ND: not detected or negative ΔCt.
  4. The temperature shows the optimal temperature from which the data were analysed.