Table 6 Results of the ligation comparison using chemicals from PPLMD I protocol with different cycle conditions

hmg

1

100

14.3 (0.4) ^a

10.0 (0.7) ^c

9.0 (1.4) ^c

12.0 (0.2)^b

12.6 (0.7)^ab

1.2E-06

2

100

13.8 (0.9) ^a

10.5 (1.0) ^cd

9.3 (0.9) ^d

12.6 (0.7) ^ab

12.0 (0.2) ^bc

6.5E-06

3

5

7.8 (1.6)

5.2 (1.3)

4.7 (2.2)

7.5 (1.1)

7.1 (0.2)

> 0.01

bar

1

0.1

ND

2

2.5

3.9 (0.5)

2.5 (0.7)

4.5 (3.19)

4.0 (0.7)

3.2 (1.0)

> 0.01

3

2.5

3.5 (1.2)

2.5 (1.3)

3.4 (3.5)

3.3 (1.3)

3.9 (0.7)

> 0.01

cry1Ab

1

0.1

ND

2

2.5

7.5 (0.0) ^b

5.2 (0.5) ^b

4.4 (2.5) ^b

11.4 (0.6) ^a

7.5 (2.3) ^b

1.2E-04

3

2.5

6.9 (0.6) ^b

5.7 (0.5) ^b

5.4 (2.4) ^b

10.9 (0.3) ^a

7.5 (2.1) ^b

5.7E04

TC 1507

1

5

ND

2

1

ND

3

2.5

ND