Table 5 Results of the ligation comparison using different chemicals with different cycle conditions

hmg

1

100

14.3 (0.4)^a

8.1 (0.7)^b

6.9 (0.4) ^bc

3.8 (3.8) ^c

5.7E-05

2

100

13.8 (0.9) ^a

8.1 (0.3) ^b

6.4 (0.4) ^bc

3.3 (3.5) ^c

2.6E-05

3

5

7.8 (1.6)

ND

ND

ND

-

bar

1

0.1

ND

-

2

2.5

3.9 (0.5)

4.5 (2.3)

1.0 (0.4)

ND

> 0.01

3

2.5

3.5 (1.2) ^ab

4.8 (2.3) ^a

0.8 (0.6) ^b

0.6 (0.5) ^b

2.3E-03

cry1Ab

1

0.1

ND

-

2

2.5

7.5 (0.0) ^a

8.2 (0.1) ^a

3.2 (1.0) ^b

0.5 (0.2) ^c

1.1E-10

3

2.5

6.9 (0.5) ^b

8.3 (0.7) ^a

3.4 (0.6) ^d

0.6 (0.4) ^d

6.3E-10

TC 1507

1

5

ND

-

2

1

ND

3

2.5

ND