Skip to main content

Table 5 Results of the ligation comparison using different chemicals with different cycle conditions

From: Comparison and transfer testing of multiplex ligation detection methods for GM plants

    ΔCt (SD)  
Target GM mixes Target % PPLMD I - 65°C DTM - 63.6°C LPA - 62.4°C PPLMD II - 62.5°C p-value ANOVA
hmg 1 100 14.3 (0.4)a 8.1 (0.7)b 6.9 (0.4) bc 3.8 (3.8) c 5.7E-05
  2 100 13.8 (0.9) a 8.1 (0.3) b 6.4 (0.4) bc 3.3 (3.5) c 2.6E-05
  3 5 7.8 (1.6) ND ND ND -
bar 1 0.1 ND -
  2 2.5 3.9 (0.5) 4.5 (2.3) 1.0 (0.4) ND > 0.01
  3 2.5 3.5 (1.2) ab 4.8 (2.3) a 0.8 (0.6) b 0.6 (0.5) b 2.3E-03
cry1Ab 1 0.1 ND -
  2 2.5 7.5 (0.0) a 8.2 (0.1) a 3.2 (1.0) b 0.5 (0.2) c 1.1E-10
  3 2.5 6.9 (0.5) b 8.3 (0.7) a 3.4 (0.6) d 0.6 (0.4) d 6.3E-10
TC 1507 1 5 ND -
  2 1 ND  
  3 2.5 ND  
  1. SD: standard deviation of the ΔCt values
  2. Values marked with different superscript characters indicate groups (for the same target-mix combination) that are significantly different according to Tukeys HSD test (P < 0.05); xa is different from yb, z abis not different from either.
  3. ND: not detected or negative ΔCt.
  4. The temperature shows the optimal temperature from which the data were analysed.