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Table 4 Differences among ligation methods used for the ligation comparison

From: Comparison and transfer testing of multiplex ligation detection methods for GM plants

   Ligation temperature (°C)
Chemicals Cycle program Tested range Published
DTM [17] DTM 46-65* 50
PPLMD II [10] PPLMD II 59.3-75* 72
LPA [12] LPA H:51.7-65*; L:54 H:60 / L:54
PPLMD I [8] PPLMD I 53-68* 65
PPLMD I DTM 50; 55.5; 59.3; 65 50
  PPLMD II 61; 65; 67.8; 72 72
  LPA H:60 / L:54 H:60 / L:54
   H:71 / L65  
   H:65 / L:59  
   H65 / L:65  
  SNPlex [15] Ramp 1: 60-51 3% Ramp: 60-51
   Ramp 2: 74-65  
   Ramp 3: 70-61  
  1. * Range with eight different temperatures. H: hybridisation, L: ligation