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Table 4 Differences among ligation methods used for the ligation comparison

From: Comparison and transfer testing of multiplex ligation detection methods for GM plants

  

Ligation temperature (°C)

Chemicals

Cycle program

Tested range

Published

DTM [17]

DTM

46-65*

50

PPLMD II [10]

PPLMD II

59.3-75*

72

LPA [12]

LPA

H:51.7-65*; L:54

H:60 / L:54

PPLMD I [8]

PPLMD I

53-68*

65

PPLMD I

DTM

50; 55.5; 59.3; 65

50

 

PPLMD II

61; 65; 67.8; 72

72

 

LPA

H:60 / L:54

H:60 / L:54

  

H:71 / L65

 
  

H:65 / L:59

 
  

H65 / L:65

 
 

SNPlex [15]

Ramp 1: 60-51

3% Ramp: 60-51

  

Ramp 2: 74-65

 
  

Ramp 3: 70-61

 
  1. * Range with eight different temperatures. H: hybridisation, L: ligation
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BMC Biotechnology

ISSN: 1472-6750

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