Skip to main content

Table 1 Final composition of the prepared GM mixes

From: Comparison and transfer testing of multiplex ligation detection methods for GM plants

Experiment Name Component Source Code Final concentration* GMO construct copy numbers in 200 ng DNA**
Ligation comparison GM mix 1 1% Bt176 maize IRMM ERM-BF411d 0.1% Bt176 18
   10% TC1507 maize IRMM ERM-BF418d 5% TC1507 889
   0% MON810 maize*** IRMM ERM-BF413a   
  GM mix 2 5% Bt176 maize IRMM ERM-BF411f 2.5% Bt176 444
   10% TC1507 maize IRMM ERM-BF418d 1% TC1507 178
   0% MON810 maize*** IRMM ERM-BF413a   
  GM mix 3 100% Bt176 maize RIKILT   2.5% Bt176 444
   100% TC1507 maize RIKILT   2.5% TC1507 444
   0% GTS 40-3-2 **** IRMM ERM-BF410a   
Specificity testing Mix 1 > 97.9% 281-24-236x3006-210-23 cotton IRMM ERM-BF422b 16.7% 6789
   100% LL25 cotton AOCS 0306-D 16.7% 6789
   100% RF3 canola AOCS 0306-G 16.7% 13047
   100% LL62 rice AOCS 0306-I 16.7% 37111
   > 89.9% MON863xNK603xMON810 maize AOCS 0406-C 16.7% 2966
   > 99.4% MON15985xMON1445 cotton AOCS 0804-F 16.7% 6789
  Mix 2 oat Biolytix   33.3%  
   barley Biolytix   33.3%  
   wheat Biolytix   33.3%  
Exchange study 1% GM mix 5% Bt176 maize IRMM ERM-BF411f 1% Bt176 178
   10% TC1507 maize IRMM ERM-BF418d 1% TC1507 178
   10% MON863 maize IRMM ERM-BF416d 1% MON863 178
   0% MON810 maize*** IRMM ERM-BF413a   
  0.1% GM mix 1% Bt176 maize 1% GM mix   0.1% Bt176 18
   1% TC1507 maize 1% GM mix   0.1% TC1507 18
   1% MON863 maize 1% GM mix   0.1% MON863 18
   0% MON810 maize*** IRMM ERM-BF413a   
  0% GM mix 0% MON810 maize*** IRMM ERM-BF413a   
  1. *expressed as percentage of total mass
  2. **based on information in the reference material certificate, unless stated otherwise in the certificate, GMO constructs were assumed to be present as single copy, homozygous insertions in the native genomic DNA
  3. ***certified as < 0.2% MON810
  4. ****certified as < 0.3% GTS 40-3-2, commercially known as Roundup Ready soy.