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Table 1 Final composition of the prepared GM mixes

From: Comparison and transfer testing of multiplex ligation detection methods for GM plants

Experiment

Name

Component

Source

Code

Final concentration*

GMO construct copy numbers in 200 ng DNA**

Ligation comparison

GM mix 1

1% Bt176 maize

IRMM

ERM-BF411d

0.1% Bt176

18

  

10% TC1507 maize

IRMM

ERM-BF418d

5% TC1507

889

  

0% MON810 maize***

IRMM

ERM-BF413a

  
 

GM mix 2

5% Bt176 maize

IRMM

ERM-BF411f

2.5% Bt176

444

  

10% TC1507 maize

IRMM

ERM-BF418d

1% TC1507

178

  

0% MON810 maize***

IRMM

ERM-BF413a

  
 

GM mix 3

100% Bt176 maize

RIKILT

 

2.5% Bt176

444

  

100% TC1507 maize

RIKILT

 

2.5% TC1507

444

  

0% GTS 40-3-2 ****

IRMM

ERM-BF410a

  

Specificity testing

Mix 1

> 97.9% 281-24-236x3006-210-23 cotton

IRMM

ERM-BF422b

16.7%

6789

  

100% LL25 cotton

AOCS

0306-D

16.7%

6789

  

100% RF3 canola

AOCS

0306-G

16.7%

13047

  

100% LL62 rice

AOCS

0306-I

16.7%

37111

  

> 89.9% MON863xNK603xMON810 maize

AOCS

0406-C

16.7%

2966

  

> 99.4% MON15985xMON1445 cotton

AOCS

0804-F

16.7%

6789

 

Mix 2

oat

Biolytix

 

33.3%

 
  

barley

Biolytix

 

33.3%

 
  

wheat

Biolytix

 

33.3%

 

Exchange study

1% GM mix

5% Bt176 maize

IRMM

ERM-BF411f

1% Bt176

178

  

10% TC1507 maize

IRMM

ERM-BF418d

1% TC1507

178

  

10% MON863 maize

IRMM

ERM-BF416d

1% MON863

178

  

0% MON810 maize***

IRMM

ERM-BF413a

  
 

0.1% GM mix

1% Bt176 maize

1% GM mix

 

0.1% Bt176

18

  

1% TC1507 maize

1% GM mix

 

0.1% TC1507

18

  

1% MON863 maize

1% GM mix

 

0.1% MON863

18

  

0% MON810 maize***

IRMM

ERM-BF413a

  
 

0% GM mix

0% MON810 maize***

IRMM

ERM-BF413a

  
  1. *expressed as percentage of total mass
  2. **based on information in the reference material certificate, unless stated otherwise in the certificate, GMO constructs were assumed to be present as single copy, homozygous insertions in the native genomic DNA
  3. ***certified as < 0.2% MON810
  4. ****certified as < 0.3% GTS 40-3-2, commercially known as Roundup Ready soy.