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Table 1 Optimized DpnI digestion time for ABI-REC

From: An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

Vector Insert DpnI-1 h DpnI-2 h
   Sequenced Confirmed Positive rate Sequenced Confirmed Positive rate
pGL3-MSTN-3.8 kb 3.8 kb 11 10 91% 10 10 100%
pGL3-MSTN-2.3 kb 2.3 kb 8 8 100% 13 13 100%
pGL3-3′ UTR 1.4 kb 9 8 89% 9 9 100%
Mean cloning efficiency   93%   100%