Table 1 Optimized DpnI digestion time for ABI-REC
From: An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy
Vector | Insert | DpnI-1 h | DpnI-2 h | ||||
|---|---|---|---|---|---|---|---|
Sequenced | Confirmed | Positive rate | Sequenced | Confirmed | Positive rate | ||
pGL3-MSTN-3.8 kb | 3.8 kb | 11 | 10 | 91% | 10 | 10 | 100% |
pGL3-MSTN-2.3 kb | 2.3 kb | 8 | 8 | 100% | 13 | 13 | 100% |
pGL3-3′ UTR | 1.4 kb | 9 | 8 | 89% | 9 | 9 | 100% |
Mean cloning efficiency | 93% | 100% | |||||