Figure 5

JMC09349 has a broad pH optimum and moderate thermal stability. A – JMC09349 has a pH optimum of 6.5 and maintains significant α-fucosidase activity between pH 3–8.5. B – JMC09349 enzymatic activity is thermo-tolerant up to 50°C for 20 min. C – DSF of purified JMC09349 gives an estimated T_M of 50.3°C. A representative plot from triplicate experiments is shown for purified JMC09349 and a buffer control.