Analyses of DAT expression independent transgenic DAT C . roseus using southern-blot and real-time PCR. A. Southern blot analysis of four randomly selected DAT transformants. Genomic DNA of transformants (1–4), the wild-type DNA (W1, W2) and the plasmid P2301 + DAT (P) digested with BamHI were used in southern blot analysis. M, λ-Hind β Marker. B. Real-time PCR analysis for the expression level of DAT gene in kanamycin-resistant C. roseus plants regenerated by transformation with pCAMBIA2301::p35s-DAT-nos. d16-d44, DAT transgenic plants; ck, average content for 15 control plants. The experiments were repeated at least 8 times and the same results were obtained.