Analysis of transgenic GUS C. roseus . A-D Histochemical GUS expression in transgenic GUS plants of regenerated C. roseus. A, C-left, D-right: non-transformed control; B, C-right and D-left: transgenic plant. Scale bar = 25.1 μm in A and B, 0.5 cm in C and 1 cm in D. E Detection of GUS gene in kanamycin-resistant C. roseus plants using PCR. M, DL2000 DNA ladder (Maker from TaKaRa); 4–9, regenerated transgenic plant; 1, DNA from non-transformant (negative control); 2, pCAMBIA2301 was used as template (positive control); 3, H2O as template (negative control). F Southern-blot analysis of transgenic C. roseus plants. 1 is untransformed control plant; 2 and 3 are the DNA from two independed transformants digested by HindIII, respectively. M, λ-Hind III Marker.