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Table 2 Sample preparation procedure and 454 sequencing results

From: Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Sample ID

Preparation steps

# raw reads

# reads in contigs

# contigs

# singletons

% globin reads

A1

ribo-minus > 1st & 2nd strand cDNA synthesis > NORM > 2nd amp

30,145

9,138

1,066

19,205

5.46

A2

wholeRNA > 1st&2nd strand cDNA synthesis > 2nd amp > NORM > 3rd amp

38,601

19,318

2,286

14,079

1.95

B1

globin-minus > ribo-minus > 1st&2nd strand cDNA synthesis

21,219

13,075

275

5,987

24.10

B2

globin-minus > ribo-minus > 1st&2nd strand cDNAsynthesis > NORM > 2nd amp

17,047

4,391

309

11,168

0.35

  1. # counted.
  2. Abbreviations used for preparation steps are explained in the Supplementary Information Additional files.