Sample ID | Preparation steps | # raw reads | # reads in contigs | # contigs | # singletons | % globin reads |
---|---|---|---|---|---|---|
A1 | ribo-minus > 1st & 2nd strand cDNA synthesis > NORM > 2nd amp | 30,145 | 9,138 | 1,066 | 19,205 | 5.46 |
A2 | wholeRNA > 1st&2nd strand cDNA synthesis > 2nd amp > NORM > 3rd amp | 38,601 | 19,318 | 2,286 | 14,079 | 1.95 |
B1 | globin-minus > ribo-minus > 1st&2nd strand cDNA synthesis | 21,219 | 13,075 | 275 | 5,987 | 24.10 |
B2 | globin-minus > ribo-minus > 1st&2nd strand cDNAsynthesis > NORM > 2nd amp | 17,047 | 4,391 | 309 | 11,168 | 0.35 |