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Table 2 Sample preparation procedure and 454 sequencing results

From: Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Sample ID Preparation steps # raw reads # reads in contigs # contigs # singletons % globin reads
A1 ribo-minus > 1st & 2nd strand cDNA synthesis > NORM > 2nd amp 30,145 9,138 1,066 19,205 5.46
A2 wholeRNA > 1st&2nd strand cDNA synthesis > 2nd amp > NORM > 3rd amp 38,601 19,318 2,286 14,079 1.95
B1 globin-minus > ribo-minus > 1st&2nd strand cDNA synthesis 21,219 13,075 275 5,987 24.10
B2 globin-minus > ribo-minus > 1st&2nd strand cDNAsynthesis > NORM > 2nd amp 17,047 4,391 309 11,168 0.35
  1. # counted.
  2. Abbreviations used for preparation steps are explained in the Supplementary Information Additional files.