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Table 1 RNA integrity, yields and DNA contamination for each tested preservation buffer and extraction method

From: Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Approach Preservation buffer Extraction kit/protocol RIN RNA yield range (μg/ 500 μl blood) 260/280 260/230 DNA contamination
% Amplification1 % Individuals with min. 1 amplicon2
Whole blood RNAlater® RiboPure™ 4.6± 2.3 5.0- 43.9 1.9± 0.1 2.1± 0.2 74.0 85.0
RNAprotect® RNeasy® 6.9± 2.6 0.0- 6.2 2.1± 0.2 0.9± 0.6 40.0 80.0
PAXgene™ PAXgene™ 7.7± 1.2 0.1- 10.2 2.1± 0.2 1.2± 0.6 54.4 95.0
TRIzol® LS TRIzol® LS 6.2± 2.9 0.2- 15.1 1.9± 0.1 1.3± 0.5 43.8 80.0
Filtered blood RNAlater® LeukoLOCK™ 7.6± 1.9 0.1- 3.7 2.0± 0.1 0.8± 0.5 11.0 20.0
  1. 1Percentage of PCRs that resulted in positive amplification. Each PCR performed in four reactions that amplified were counted.
  2. 2 Percentage of individuals in which a minimum of a single amplification occurred.