Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Table 1 RNA integrity, yields and DNA contamination for each tested preservation buffer and extraction method

Approach	Preservation buffer	Extraction kit/protocol	RIN	RNA yield range (μg/ 500 μl blood)	260/280	260/230	DNA contamination
Approach	Preservation buffer	Extraction kit/protocol	RIN	RNA yield range (μg/ 500 μl blood)	260/280	260/230	% Amplification¹	% Individuals with min. 1 amplicon²
Whole blood	RNAlater®	RiboPure™	4.6± 2.3	5.0- 43.9	1.9± 0.1	2.1± 0.2	74.0	85.0
	RNAprotect®	RNeasy®	6.9± 2.6	0.0- 6.2	2.1± 0.2	0.9± 0.6	40.0	80.0
	PAXgene™	PAXgene™	7.7± 1.2	0.1- 10.2	2.1± 0.2	1.2± 0.6	54.4	95.0
	TRIzol® LS	TRIzol® LS	6.2± 2.9	0.2- 15.1	1.9± 0.1	1.3± 0.5	43.8	80.0
Filtered blood	RNAlater®	LeukoLOCK™	7.6± 1.9	0.1- 3.7	2.0± 0.1	0.8± 0.5	11.0	20.0

¹Percentage of PCRs that resulted in positive amplification. Each PCR performed in four reactions that amplified were counted.
² Percentage of individuals in which a minimum of a single amplification occurred.

ISSN: 1472-6750