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Table 1 Percentage of plasmid injected zebrafish expressing GFP in different body tissues after exposure to oestrogens (at 72 hpf)

From: Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos

 

Control

EE2 (ng/L)

E2 (ng/L)

NP (μg/L)

1000

100

10

1000

100

10

Liver

0

74.3% (223/300)

61.6% (185/300)

0

70.7% (212/300)

18.7% (56/300)

0

Heart

0

82.7% (248/300)

66.3% (199/300)

0

78% (234/300)

65% (195/300)

56% (168/300)

Muscle

13.3% (40/300)

90.3% (271/300)

78.3% (235/300)

27.3% (82/300)

87% (261/300)

74% (222/300)

71.3% (214/300)

Forebrain

 

33% (99/300)

0

0

25.6% (77/300)

0

0

Otic vesicle

19% (47/300)

26.3% (78/300)

16.6% (50/300)

10.6% (32/300)

27% (81/300)

25.3% (76/300)

0

lateral line neuromast

10% (32/300)

22.3% (67/300)

13% (39/300)

14.7% (44/300)

25.3% (76/300)

18% (54/300)

4%

(12/300)