Figure 3From: Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryosComparison of detectable response concentrations for different oestrogen compounds in the zebrafish embryo transient assay. Injected embryos were exposed for up to 72 h post-fertilisation to 17β-oestradiol (E2) and nonylphenol (NP) and fluorescence detected using fluorescence microscopy (Leica DMI 4000 B). No GFP expression was observed in controls (A1-A3), but low level GFP expression occurred in the liver for exposure to 1000 ng E2/L (C2) and high level GFP expression was detected in the somite muscle and heart at exposure concentrations of 100 and 1000 ng E2/L. Weak GFP expression was also detected in the heart and somite muscle of embryos for exposure to 10 μg/L NP (D1-D3). Embryos exposed to the higher concentrations of NP (100 μg/L) died. This experiment was run in duplicate and was repeated at least seven times.Back to article page