Figure 3

Verification of Bax, catalase or p53 cDNA insertion into pBLIC. Clones were digested with HindIII and run on a 0.7% agarose gel. A 1 kb ladder is used to mark band sizes. Gels were photographed on a Canon Powershot G10 mounted on a UVP Multidoc-it Digital Imaging System using standard preset contrast settings. The mobility shift relative to the empty pBLIC vector indicates presence of the cDNA insert. Restriction digest products for (A) pBLIC-Bax, (B) pBLIC-catalase, and (C) pBLIC-p53 are shown. Note that the shift in HindIII-treated plasmids is greater for catalase and p53 relative to Bax, corresponding to the larger insert size of the former two cDNAs relative to the latter (catalase: 1584 bp, p53: 1182 bp vs. Bax: 657 bp).