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Figure 4 | BMC Biotechnology

Figure 4

From: Identification of immunogenic proteins and generation of antibodies against SalmonellaTyphimurium using phage display

Figure 4

ELISA for analysis of identified immunogenic Salmonella Typhimurium proteins with defined pig sera. 1: putative dihydroyacid dehydratase; 2: putative electron transfer protein alpha; 3: 2,4-dienyl-CoA reductase, 4: phage tail like protein; 5: putative dimethyl sulphoxide reductase. The sera from Salmonella-positive pigs (according to „Pig Screen“ ELISA) are marked dark blue, the sera from Salmonella-negative pigs (according to „Pig Screen“ ELISA) are marked orange, the mixture of positive sera used for the selection of immunogenic proteins is marked green, the piglet serum is marked dark red and the detection system control (only detection antibodies) is marked in light blue. For the panel of positive and negative sera arithmetic mean and standard deviation are given as black lines. 1 μg purified antigens were coated. The antigens were detected with diluted swine sera (1:200 in 2% MPBST) and goat anti-swine IgG HRP conjugate (1:10,000)

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